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Journal: Structure (London, England : 1993)
Article Title: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability
doi: 10.1016/j.str.2022.06.004
Figure Lengend Snippet: Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also
Article Snippet: Select hybridomas were sent to
Techniques: Sequencing, Binding Assay, Isolation, Enzyme-linked Immunosorbent Assay, Purification, Bioprocessing, Neutralization